gaaaaah

[moonwise]

On the day they gave out brains, I was last in line.

Okay, literature gradient conditions say "30% 0.1% TFA/acetonitrile to 40%."

First I leave out the TFA part. (TFA = trifluoroacetic acid)

Then I put in the TFA part, but I put the TFA in acetonitrile. Wrooooooong.

Ok, put TFA into water, like it's supposed to be. Still getting no peaks. WTF.

Then I discovered:

It's 30% WATER/TFA as the starting conditions, not 30% ACETONITRILE

DUMB DUMB DUMBASS

NO WONDER MY PEAKS ARE ROCKETING OFF THE FUCKING COLUMN

dammit.

Comments

[tntoxfox.livejournal.com]

We work with a Shimadzu. Decent machine, but the software for it is a pain in the butt to work with. Not intuitive at all. The machine also didn't beep when one of the solvents ran dry, so you have to keep a close eye on those.