gaaaaah

[moonwise]

On the day they gave out brains, I was last in line.

Okay, literature gradient conditions say "30% 0.1% TFA/acetonitrile to 40%."

First I leave out the TFA part. (TFA = trifluoroacetic acid)

Then I put in the TFA part, but I put the TFA in acetonitrile. Wrooooooong.

Ok, put TFA into water, like it's supposed to be. Still getting no peaks. WTF.

Then I discovered:

It's 30% WATER/TFA as the starting conditions, not 30% ACETONITRILE

DUMB DUMB DUMBASS

NO WONDER MY PEAKS ARE ROCKETING OFF THE FUCKING COLUMN

dammit.

Comments

[arafel.livejournal.com]

*grins* Losing prime is a minor infraction. Recite the periodic table and your sins will be forgiven. Do you work with an Agilent? Waters instruments at least will shut down when they lose prime, and they don't pump your column dry.

[tntoxfox.livejournal.com]

We work with a Shimadzu. Decent machine, but the software for it is a pain in the butt to work with. Not intuitive at all. The machine also didn't beep when one of the solvents ran dry, so you have to keep a close eye on those.