gaaaaah

[moonwise]

On the day they gave out brains, I was last in line.

Okay, literature gradient conditions say "30% 0.1% TFA/acetonitrile to 40%."

First I leave out the TFA part. (TFA = trifluoroacetic acid)

Then I put in the TFA part, but I put the TFA in acetonitrile. Wrooooooong.

Ok, put TFA into water, like it's supposed to be. Still getting no peaks. WTF.

Then I discovered:

It's 30% WATER/TFA as the starting conditions, not 30% ACETONITRILE

DUMB DUMB DUMBASS

NO WONDER MY PEAKS ARE ROCKETING OFF THE FUCKING COLUMN

dammit.