gaaaaah

[moonwise]

On the day they gave out brains, I was last in line.

Okay, literature gradient conditions say "30% 0.1% TFA/acetonitrile to 40%."

First I leave out the TFA part. (TFA = trifluoroacetic acid)

Then I put in the TFA part, but I put the TFA in acetonitrile. Wrooooooong.

Ok, put TFA into water, like it's supposed to be. Still getting no peaks. WTF.

Then I discovered:

It's 30% WATER/TFA as the starting conditions, not 30% ACETONITRILE

DUMB DUMB DUMBASS

NO WONDER MY PEAKS ARE ROCKETING OFF THE FUCKING COLUMN

dammit.

Comments

[arafel.livejournal.com]

Eh, it turned out that the "right" way to run the gradient was as crappy-looking as the "wrong" way to run it. Now we're into method development. The joy.

And yes, I feel your pain on the "omg look how well I did" thing. Trust me, it ain't often that I'm not reminded of how not-brilliant I can be...